yrkA [2019-08-28 09:16:41]

similar to magnesium exporter

locus: BSU_26610

pI: 4.92

mw: 48.82 kDa

protein length: 434 aa

gene length: 1305 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,718,959 2,720,263

The protein

Protein family

• [SW|UPF0053 family](according to UniProt)

Paralogous protein(s)

• [protein|A459410312A926365B45CEB4694DE399B38820A2|YugS], [protein|B2E9D026B668C8530C406353E3400059388566FB|YhdT], [protein|BE0777DE7C40825D4DB7252EA84AAB3892578529|MpfA], [protein|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|YqhB]

[SW|Domains]

• [SW|DUF21 domain] (5-201)
• 2 [SW|CBS domain]s (220-282, 289-346)

Structure

• [PDB|4HG0] (CorC from E. coli, corresponds to the [SW|CBS domain]s and the C-terminal transporter-associated domain)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE26610 ([gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGATTTGCCTTACCTAG, downstream forward: _UP4_TAACATCTGGATAAAACACA
• BKK26610 ([gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGATTTGCCTTACCTAG, downstream forward: _UP4_TAACATCTGGATAAAACACA

Expression vectors

• pGP2932 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

• 27933050,31415562